Notes
Outline
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MALDI: Matrix Assisted Laser Desorption Ionization
5
Desorption / Ionization
Matrix-assisted Laser
Desorption / Ionization
Surface of the Target
Time-of-Flight Analyzer
Slide 37
Calculation of the Time-of-Flight
Slide 39
Linear Mode
High mass (above 50 kDa)
High sensitivity
Delayed Extraction (DE) Mode
Low mass (below 40 kDa)
High resolution
High sensitivity
Reflector Mode
Low mass (below 10 kDa)
High resolution
Low sensitivity
Post Source Decay (PSD)
Low mass (below 3 kDa)
Structural information
Matrix for 337 nm laser light
Matrix for 337 nm laser light
Loading Methods
Thin Layer Method
Matrix Crystallization After Drying
Buffers
Substance P in HCCA
Bovine Insulin in Sinapinic Acid
Mixture of 3 Proteins in SA
Mixture of Trypsin and Trypsinogen
Mixture of Trypsin and Trypsinogen
Colon Tumor Extract in SA
Colon Tumor Extract in SA
Influence of the Choice of Matrix
IgG in Sinapinic Acid
MALDI-TOF Mass Spectrometry
Glycoprotein in Sinapinic Acid
Cox2 in Sinapinic Acid (Glycoprotein)
Peptide sequencing : Enzymatic
Post-Source
Decay (PSD)
MALDI,
Principle
PSD Example
Protein Identification by MALDI-MS
Peptide Mapping
After Tryptic Digest
Peptide mass Fingerprinting
Example of Fingerprinting
Conclusions
Slide 70
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Notes